Md. Mahmudul Hasan

Directed molecular biology assay development team, focusing on conceptualising and validating innovative qPCR methods for HLB bacteria detection in citrus. Developed and standardised high-throughput DNA/RNA extraction and purification protocol, enhancing efficiency in citrus sample processing. Screened ~5000 citrus samples from field trials to detect HLB with an improved qPCR method. Supported other project team to develop antimicrobial peptides to control citrus greening disease. Troubleshot technical challenges to maintain project momentum and continuity. Analysed experimental data, synthesised findings, and communicated results to project teams and external stakeholders, ensuring clear understanding of research progress and outcomes.

• Conceptualised and validated a viability qPCR method, significantly improving HLB bacteria detection in citrus.
• Developed a protocol for high-throughput DNA extraction and purification and PCR detection of HLB bacteria causing Citrus greening disease.
• Designed and constructed different RNAi (dsRNA, Dumbbell RNA) constructs for controlling crop insect pest.
• Analysed gene expression from RNAi-fed insects (Colorado potato beetle, Diamond back moth).

Conducted identification and characterisation of three plant-derived small-secreted effector protein molecules involved in plant fungal symbiosis Tested efficacy of DNA delivery to plants and fungi utilising cell-penetrating peptides. Formulated comprehensive guidelines for construct design tailored to CRISPR/Cas-based gene editing in plants. Designed and constructed plasmids essential for CRISPR/Cas-based genome editing in plants. Designed and fabricated plasmids to facilitate creation of a novel split-base editor for plants. Designed, built, and tested synthetic CO2 fixation pathways in Arabidopsis and E. coli. Conducted genome-wide analysis to identify and characterise silicon transporter genes in poplar. Compiled progress reports and delivered presentations to funding agency to showcase research advancements and achievements.

• Uncovered a novel nuclear localisation signal sequence, expanding understanding of cellular trafficking mechanisms.
• Identified and characterised multiple silicon transporter genes in bioenergy crop poplar.
• Utilised advanced machine learning to identify and characterise a cell-penetrating peptide from plant-secreted effector proteins.
• Developed gene editing protocol for non-model plant pathogenic fungi Sphaerulina musiva.
• Published 15 peer-reviewed journal articles, establishing a strong presence in scientific community.
• Supervised a PhD student's research on decoding molecular mechanism of symbiosis between Laccaria bicolour and poplar.
• Developed more than 200 plasmid constructs throughout postdoctoral tenure.
• Directed setup of state-of-the-art molecular biology laboratory for host group.

Deliver comprehensive lectures and practical sessions in Molecular Genetics at undergraduate level and Plant Biotechnology at graduate level. Design and supervise laboratory exercises in cellular and molecular biology, including advanced techniques such
as PCR and qRT-PCR. Provide individualised support to students to enhance their understanding and problem-solving abilities in plant genetics and biotechnology. Assess and provide feedback on assignments, tests, and laboratory work to facilitate academic development. Contribute to curriculum development and continuous improvement of educational materials and methods.

• Supervised two master's thesis projects on characterising apomictic genes in cereals.
• Secured $ 3000 grant for study of apomictic genes in cereal crops.
• Recognised as a keynote speaker at 5th International Conference on Biotechnology in Health and Agriculture, underscoring expertise and thought leadership in the field.

Facilitated the course Genetics and Evolution of Life, preparing experiments to enhance student comprehension of fundamental concepts. Guided students through intricate laboratory techniques, including gene cloning, bacterial and yeast transformation, and fluorescence protein expression in yeast. Developed instructional materials and methodologies to optimise learning experience.

Serving as Primary Supervisor for a PhD candidate researching [thesis topic, e.g., "machine learning approaches to early disease detection in livestock"]. Responsibilities include guiding research design, methodology, and data analysis; reviewing manuscript drafts for peer-reviewed publication; and mentoring the candidate through coursework, qualifying exams, and dissertation defense preparation. The candidate has published [X] papers in peer-reviewed journals during the supervision period, with the dissertation expected to be completed by [expected year].

Served as Co-Supervisor for a Master's thesis investigating [research topic, e.g., "antimicrobial resistance patterns in poultry farms across central Bangladesh"]. Provided guidance on experimental design, statistical analysis, and academic writing, with regular progress reviews throughout the research period. The thesis was successfully defended in 2023, and findings were subsequently published in a peer-reviewed journal, contributing to ongoing surveillance efforts in veterinary public health.

Regularly serves as an invited peer reviewer for several international journals, evaluating manuscript quality, methodology, and scientific rigor across the fields of plant science, genetics, ecology, and molecular biology.

Regularly serves as an invited peer reviewer for several international journals, evaluating manuscript quality, methodology, and scientific rigor across the fields of plant science, genetics, ecology, and molecular biology.